Journal: Soft matter

Article Title: Microgel Film Dynamics Modulate Cell Adhesion Behavior

doi: 10.1039/C3SM52518J

Figure Lengend Snippet: Fluorescence intensity values for fluorescent fibronectin adsorbed into microgel films at 37°C. Error bars are standard deviations. Results are statistically significant with all p < 0.0045 (n=3).

Article Snippet: Protein Adsorption Assay Human plasma fibronectin (FN, Mw: 400k – 500k, 500 μg/mL, Invitrogen) was coupled with Alexa Fluor ® 488 succinimidyl ester (1 mg/mL, Molecular Probes) in 0.1M NaHCO 3 (pH 9.0, 100 μL) for 2 hours at room temperature.

Techniques: Fluorescence

Journal: Molecular Oncology

Article Title: SERPINA5 inhibits tumor cell migration by modulating the fibronectin‐integrin β1 signaling pathway in hepatocellular carcinoma

doi: 10.1016/j.molonc.2013.12.003

Figure Lengend Snippet: Downregulation of SERPINA5 gene in HCC. (A) Determination of DNA dosages of the SERPINA5 gene by quantitative real‐time PCR (q‐PCR) in an independent cohort of 125 paired HCCs and adjacent non‐tumor tissues (NT) with genomic DNA ...

Article Snippet: Human plasma SERPINA5 is mainly synthesized in the liver ( Francis and Thomas, 1984 ), but it is also produced in kidneys and reproductive organs, including testes, seminal vesicles and ovaries ( Laurell et al., 1992 ).

Techniques: Real-time Polymerase Chain Reaction

Journal: Molecular Oncology

Article Title: SERPINA5 inhibits tumor cell migration by modulating the fibronectin‐integrin β1 signaling pathway in hepatocellular carcinoma

doi: 10.1016/j.molonc.2013.12.003

Figure Lengend Snippet: Loss of SERPINA5 expression correlates with malignant features of HCC. (A) The expression level of SERPINA5 gene in 58 paired HCC and matched non‐tumor tissues were determined by q‐PCR. The data are expressed as the log2 fold change (ΔCt ...

Article Snippet: Human plasma SERPINA5 is mainly synthesized in the liver ( Francis and Thomas, 1984 ), but it is also produced in kidneys and reproductive organs, including testes, seminal vesicles and ovaries ( Laurell et al., 1992 ).

Techniques: Expressing

Journal: Molecular Oncology

Article Title: SERPINA5 inhibits tumor cell migration by modulating the fibronectin‐integrin β1 signaling pathway in hepatocellular carcinoma

doi: 10.1016/j.molonc.2013.12.003

Figure Lengend Snippet: Targeted downregulation of SERPINA5 promotes HCC cell migration. (A) Detection of shRNA‐mediated knockdown of SERPINA5 gene in HUH‐7 and Hep3B cells by RT‐PCR. (B) Detection of shRNA‐mediated knockdown of SERPINA5 in HUH‐7 ...

Article Snippet: Human plasma SERPINA5 is mainly synthesized in the liver ( Francis and Thomas, 1984 ), but it is also produced in kidneys and reproductive organs, including testes, seminal vesicles and ovaries ( Laurell et al., 1992 ).

Techniques: Migration, shRNA, Knockdown, Reverse Transcription Polymerase Chain Reaction

Journal: Molecular Oncology

Article Title: SERPINA5 inhibits tumor cell migration by modulating the fibronectin‐integrin β1 signaling pathway in hepatocellular carcinoma

doi: 10.1016/j.molonc.2013.12.003

Figure Lengend Snippet: Overexpression of SERPINA5 inhibits HCC cell migration and metastasis. (A) The detection of lentiviral‐mediated overexpression of SERPINA5 in MHCC‐97H, SNU‐449 and HCCLM3 cells by immunoblotting. (B) Representative result of the ...

Article Snippet: Human plasma SERPINA5 is mainly synthesized in the liver ( Francis and Thomas, 1984 ), but it is also produced in kidneys and reproductive organs, including testes, seminal vesicles and ovaries ( Laurell et al., 1992 ).

Techniques: Over Expression, Migration, Western Blot

Journal: Molecular Oncology

Article Title: SERPINA5 inhibits tumor cell migration by modulating the fibronectin‐integrin β1 signaling pathway in hepatocellular carcinoma

doi: 10.1016/j.molonc.2013.12.003

Figure Lengend Snippet: Secreted SERPINA5 inhibits the migration of HCC cells. (A) Detection of the secreted SERPINA5 protein levels in seven HCC cell lines by immunoblotting. (B) Detection of the secreted SERPINA5 protein levels in MHCC‐97H, SNU‐449 and HCCLM3 ...

Article Snippet: Human plasma SERPINA5 is mainly synthesized in the liver ( Francis and Thomas, 1984 ), but it is also produced in kidneys and reproductive organs, including testes, seminal vesicles and ovaries ( Laurell et al., 1992 ).

Techniques: Migration, Western Blot

Journal: Molecular Oncology

Article Title: SERPINA5 inhibits tumor cell migration by modulating the fibronectin‐integrin β1 signaling pathway in hepatocellular carcinoma

doi: 10.1016/j.molonc.2013.12.003

Figure Lengend Snippet: The direct interaction of SERPINA5 with fibronectin. (A) Schematic model of the identification of SERPINA5‐binding proteins using LC‐MS/MS to analyze immunoprecipitated proteins, by use of antibody against the SERPINA5 in membrane and ...

Article Snippet: Human plasma SERPINA5 is mainly synthesized in the liver ( Francis and Thomas, 1984 ), but it is also produced in kidneys and reproductive organs, including testes, seminal vesicles and ovaries ( Laurell et al., 1992 ).

Techniques: Binding Assay, Liquid Chromatography with Mass Spectroscopy, Immunoprecipitation, Membrane

Journal: Molecular Oncology

Article Title: SERPINA5 inhibits tumor cell migration by modulating the fibronectin‐integrin β1 signaling pathway in hepatocellular carcinoma

doi: 10.1016/j.molonc.2013.12.003

Figure Lengend Snippet: SERPINA5 inhibits HCC cell migration by disrupting the fibronectin/integrin β1 signaling pathway. (A) Treatment of SERPINA5 significantly inhibits the migration of SNU‐449 and HCCLM3 cells, which can be restored by exogenous fibronectin ...

Article Snippet: Human plasma SERPINA5 is mainly synthesized in the liver ( Francis and Thomas, 1984 ), but it is also produced in kidneys and reproductive organs, including testes, seminal vesicles and ovaries ( Laurell et al., 1992 ).

Techniques: Migration